| EAAT5

Excitatory amino-acid transporter 5 (EAAT5) is a protein that is encoded by the SLC1A7 gene.EAAT5 is expressed predominantly in the retina, has high affinity for the excitatory amino acid L-glutamate. When stimulated by this amino acid, EAAT5 conducts chloride ions.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.25	1.51	1.02	0.48	0.33	0.24	0.19	0.09
Corrected OD	2.16	1.42	0.93	0.39	0.24	0.15	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human EAAT5 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	81-101	95

 

| Linearity

Samples were spiked with high concentrations of Human EAAT5 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)
1 : 2	Range (%)	83-95
	Average (%)	91
1 : 4	Range (%)	89-102
	Average (%)	95